pSETT4gap
(Plasmid #153413)

• Purpose: (Empty Backbone) φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette
• Depositing Lab: Joern Kalinowski
• Publication: Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(39). pii: 9/39/e00596-20. doi: 10.1128/MRA.00596-20.

Backbone

• Vector backbone: pSET152
• Backbone size (bp): 5715
• Modifications to backbone: The pSETT4 vector is integrating into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4-terminators are surrounding the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in further Actinomycetales by exchanging the promoter.
• Vector type: Bacterial Expression
• Promoter: gapDH promoter from Eggerthella lenta
• Selectable markers: Apramycin

Growth in Bacteria

• Bacterial Resistance(s): Apramycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pSETT4gap
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry