pSETT4gap
(Plasmid
#153413)
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Purpose(Empty Backbone) φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSET152
- Backbone size (bp) 5715
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Modifications to backboneThe pSETT4 vector is integrating into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4-terminators are surrounding the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in further Actinomycetales by exchanging the promoter.
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Vector typeBacterial Expression
- Promoter gapDH promoter from Eggerthella lenta
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Selectable markersApramycin
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSETT4gap was a gift from Joern Kalinowski (Addgene plasmid # 153413 ; http://n2t.net/addgene:153413 ; RRID:Addgene_153413) -
For your References section:
pSETT4, an Improved phiC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Schaffert L, Jacob L, Schneiker-Bekel S, Persicke M, Marz C, Ruckert C, Puhler A, Kalinowski J. Microbiol Resour Announc. 2020 Sep 24;9(39). pii: 9/39/e00596-20. doi: 10.1128/MRA.00596-20. 10.1128/MRA.00596-20 PubMed 32972929
Map uploaded by the depositor.
