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PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV-CMV-EF1a-IRES-Blast
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-WT-KRAS
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SpeciesH. sapiens (human)
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GenBank IDNM_033360
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Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-mCherry-WT-KRAS-IRES-Blast was a gift from Josef Penninger (Addgene plasmid # 153335 ; http://n2t.net/addgene:153335 ; RRID:Addgene_153335) -
For your References section:
Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM. Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. 10.1038/s42003-020-1072-4 PubMed 32620833
Map uploaded by the depositor.
