pCASP-SptP120-3m160-HilA
(Plasmid
#153325)
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PurposeTriggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCASP-HilA
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Vector typeSalmonella expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSptP120-3m160 DARPin
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SpeciesSynthetic
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCASP-SptP120-3m160-HilA was a gift from Josef Penninger (Addgene plasmid # 153325 ; http://n2t.net/addgene:153325 ; RRID:Addgene_153325) -
For your References section:
Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM. Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. 10.1038/s42003-020-1072-4 PubMed 32620833
Map uploaded by the depositor.
