pCASP-3G124-HilA
(Plasmid
#153324)
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PurposeShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153324 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCASP-HilA
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Vector typeSalmonella expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3G124 DARPin but no expression
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCASP-3G124-HilA was a gift from Josef Penninger (Addgene plasmid # 153324 ; http://n2t.net/addgene:153324 ; RRID:Addgene_153324) -
For your References section:
Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol. Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM. Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. 10.1038/s42003-020-1072-4 PubMed 32620833