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PurposeBacterial expression of HIV reverse transcriptase
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET30a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV Reverse Transcriptase
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SpeciesHIV
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Insert Size (bp)1716
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid made by Bryan Dickinson and Huiqing Zhou at University of Chicago
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30a_HIV-RT was a gift from Bryan Dickinson & Drew Endy (Addgene plasmid # 153311 ; http://n2t.net/addgene:153311 ; RRID:Addgene_153311)