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pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
(Plasmid #153302)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEST
  • Backbone size w/o insert (bp) 2524
  • Total vector size (bp) 3148
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    KL740
  • Growth instructions
    Critical to grow in KL740 at 29-30C, or will overexpress and mutate
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    clpP
  • Alt name
    ATP-dependent Clp protease proteolytic subunit
  • Species
    Escherichia coli
  • Insert Size (bp)
    624
  • GenBank ID
    NP_414971.1
  • Entrez Gene
    clpP (a.k.a. b0437, ECK0431, lopP)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEST_OR2-OR1-Pr-UTR1_ClpP-T500 was a gift from Mikhail Shapiro (Addgene plasmid # 153302 ; http://n2t.net/addgene:153302 ; RRID:Addgene_153302)

  • For your References section:

    Acoustic biosensors for ultrasound imaging of enzyme activity. Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG. Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. 10.1038/s41589-020-0591-0 PubMed 32661379
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