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Addgene

pTetR-BCD2-Ana GvpC
(Plasmid #153298)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTARA Addgene 31491
  • Backbone size w/o insert (bp) 4857
  • Total vector size (bp) 5439
  • Modifications to backbone
    Deletion of araBAD promoter, araC and T7 RNA polymerase, insertion of tetr/tetA promoters, TetR and BCD2 ribosome binding site upstream of the GvpC gene.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gas vesicle protein C
  • Alt name
    GvpC
  • Species
    Anabaena flos-aquae
  • Insert Size (bp)
    582
  • Promoter pTet

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTetR-BCD2-Ana GvpC was a gift from Mikhail Shapiro (Addgene plasmid # 153298 ; http://n2t.net/addgene:153298 ; RRID:Addgene_153298)

  • For your References section:

    Acoustic biosensors for ultrasound imaging of enzyme activity. Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG. Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. 10.1038/s41589-020-0591-0 PubMed 32661379
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