N-His-GvpC-ssrA
(Plasmid #153297)

• Purpose: Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5248
• Total vector size (bp): 5896
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
• Alt name: GvpC
• Species: Anabaena flos-aquae
• Insert Size (bp): 648
• Mutation: 1) N-terminal 6xHis tag flanked by 1x glycine residues 2) 33 bp C-terminal ssrA tag linked to the GvpC sequence by a 2 amino acid 'SG' linker
• Promoter: T7
• Tags / Fusion Proteins:
  • His-tag (N terminal on insert)
  • ssrA tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 terminator reverse

Resource Information

• Supplemental Documents:
  • N-His-GvpC-SG-ssrA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry