C-His-GvpC-TEV
(Plasmid #153296)

• Purpose: Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13.

Backbone

• Vector backbone: pET26b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5207
• Total vector size (bp): 5828
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TEV-sensitive Gas vesicle protein C (his-tagged)
• Alt name: GvpC
• Species: Anabaena flos-aquae
• Insert Size (bp): 621
• Mutation: 1) 21 bp TEV recognition and cleavage site flanked by GSGSGSG linkers, 3) C-terminal His-tag linked to the GvpC sequence with a 3 amino acid (SLE) linker
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 terminator reverse

Resource Information

• Supplemental Documents:
  • Ana GvpC with TEV cleavage site.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry