FLAG-MRGBP
(Plasmid
#15325)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Hyg(+)
- Backbone size w/o insert (bp) 5601
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMRG-binding protein
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Alt nameMRGBP
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SpeciesH. sapiens (human)
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Insert Size (bp)615
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Entrez GeneMRGBP (a.k.a. C20orf20, Eaf7, MRG15BP, URCC4)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-MRGBP was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15325 ; http://n2t.net/addgene:15325 ; RRID:Addgene_15325) -
For your References section:
Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex. Cai Y, Jin J, Tomomori-Sato C, Sato S, Sorokina I, Parmely TJ, Conaway RC, Conaway JW. J Biol Chem. 2003 Oct 31. 278(44):42733-6. 10.1074/jbc.C300389200 PubMed 12963728