pAGM55273
(Plasmid
#153211)
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Purpose(Empty Backbone) Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAGM4723
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Backbone manufacturerMarillonnet, IPB
- Backbone size (bp) 4824
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM55273 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153211 ; http://n2t.net/addgene:153211 ; RRID:Addgene_153211) -
For your References section:
High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100135 PubMed 33898975
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