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Addgene

Paxillin-pEGFP
(Plasmid #15233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    paxillin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1700
  • GenBank ID
    L30099
  • Entrez Gene
    PXN (a.k.a. TGFB1I1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Kpn (not destroyed)
  • 5′ sequencing primer CMV immediate early gene forward primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Paxillin-pEGFP was a gift from Rick Horwitz (Addgene plasmid # 15233 ; http://n2t.net/addgene:15233 ; RRID:Addgene_15233)

  • For your References section:

    Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. Laukaitis CM, Webb DJ, Donais K, Horwitz AF. J Cell Biol. 2001 Jun 25. 153(7):1427-40. 10.1083/jcb.153.7.1427 PubMed 11425873
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