pEGFP hsp70 K71E
(Plasmid #15216)

• Depositing Lab: Lois Greene
• Publication: Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-5000.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4727
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hsp70 K71E
• Alt name: hsp70
• Species: H. sapiens (human)
• Mutation: K71E, ATPase-deficient mutant.
• GenBank ID: M11717
• Entrez Gene: HSPA1A (a.k.a. HEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C primer
• 3′ sequencing primer: SV40pA-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate the presence of an extra Arginine, R469, and mutation G370A when compared to the reference sequence M11717.