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Addgene

Cerulean
(Plasmid #15214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric Cerulean
  • Alt name
    mCER
  • Species
    synthetic
  • Insert Size (bp)
    731
  • Mutation
    A206K

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Bsr GI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The EGFP in pEGFP-C1 was replaced with Cerulean.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cerulean was a gift from Dave Piston (Addgene plasmid # 15214 ; http://n2t.net/addgene:15214 ; RRID:Addgene_15214)

  • For your References section:

    An improved cyan fluorescent protein variant useful for FRET. Rizzo MA, Springer GH, Granada B, Piston DW. Nat Biotechnol. 2004 Apr . 22(4):445-9. 10.1038/nbt945 PubMed 14990965
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