Flag-Paxillin
(Plasmid #15212)

• Depositing Lab: Rick Horwitz
• Publication: Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Paxillin
• Species: G. gallus (chicken)
• Insert Size (bp): 1700
• GenBank ID: L30099
• Entrez Gene: PXN (a.k.a. TGFB1I1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Chris Turner SUNY Upstate Medical
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry