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pRAVE-nLacZ (CC#111)
(Plasmid #15135)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRAVE
  • Backbone manufacturer
    Cepko Lab
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nLacZ
  • Alt name
    nuclear LacZ

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In addition to the long terminal repeat (LTR) promoter, the RAVE-nlacZ viral
vector has an internal Xenopus elongation factor alpha (EF1-alpha) promoter. To construct RAVE-nlacZ, the Xenopus EF1-alpha gene from pEF-puro (from Gord Fishell), was cloned into the pRIA vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRAVE-nLacZ (CC#111) was a gift from Connie Cepko (Addgene plasmid # 15135 ; http://n2t.net/addgene:15135 ; RRID:Addgene_15135)

  • For your References section:

    The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments. Peters MA, Cepko CL. Dev Biol. 2002 Nov 1. 251(1):59-73. 10.1006/dbio.2002.0791 PubMed 12413898
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