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Addgene

pHes1-GFP (CC#109)
(Plasmid #15133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-MN-IRES-E-GFP
  • Backbone manufacturer
    Gary Nolan, Stanford
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow at 30C in Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hes1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1100
  • Entrez Gene
    Hes1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An EcoRI-AhaIII fragment containing the entire Hes1 coding region was blunted and subcloned in the blunted BamHI site of the pGFP virus vector.

Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHes1-GFP (CC#109) was a gift from Connie Cepko (Addgene plasmid # 15133 ; http://n2t.net/addgene:15133 ; RRID:Addgene_15133)

  • For your References section:

    rax, Hes1, and notch1 promote the formation of Muller glia by postnatal retinal progenitor cells. Furukawa T, Mukherjee S, Bao ZZ, Morrow EM, Cepko CL. Neuron. 2000 May . 26(2):383-94. 10.1016/S0896-6273(00)81171-X PubMed 10839357
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