pC.HA
(Plasmid
#15126)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCF1
- Backbone size (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pC.HA was derived from plasmid pCG (Tanaka, M, and Herr, W., 1990. Cell 60:375-386). pCG contains the human CMV promoter, the HSV tk gene 5' untranslated leader and initiation codon, rabbit beta-globin gene splicing and polyA singals, and the replicon origin of SV40.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC.HA was a gift from Joan Brugge (Addgene plasmid # 15126 ; http://n2t.net/addgene:15126 ; RRID:Addgene_15126) -
For your References section:
Vav family proteins couple to diverse cell surface receptors. Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, Brugge JS, Swat W. Mol Cell Biol. 2000 Sep . 20(17):6364-73. 10.1128/MCB.20.17.6364-6373.2000 PubMed 10938113