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M76 pIRESpuro-GLUE-hDsh2
(Plasmid #15101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro-GLUE
  • Backbone manufacturer
    Moon Lab
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dsh2
  • Alt name
    Dishevelled 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    DVL2
  • Tags / Fusion Proteins
    • streptag (N terminal on backbone)
    • HA (N terminal on backbone)
    • CBP tag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M76 pIRESpuro-GLUE-hDsh2 was a gift from Randall Moon (Addgene plasmid # 15101 ; http://n2t.net/addgene:15101 ; RRID:Addgene_15101)

  • For your References section:

    The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation. Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss MJ, Moon RT. Nat Cell Biol. 2006 Apr . 8(4):348-57. 10.1038/ncb1381 PubMed 16547521
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