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Addgene

pMV2 BMPR (DM#138)
(Plasmid #15069)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV2
  • Backbone size w/o insert (bp) 4200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP receptor
  • Alt name
    BMPR
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1500
  • Entrez Gene
    bmpr1a (a.k.a. acvrlk3, alk-3, alk3, cd292, skr5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMV2, derivative of pMT3, is a mammalian expression vector. This construct conatins an EcoRI-SalI insert of the p64TNE BMPR clone with no untranslated region. The vector has an SV40 origin and an Ad MLP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMV2 BMPR (DM#138) was a gift from Douglas Melton (Addgene plasmid # 15069 ; http://n2t.net/addgene:15069 ; RRID:Addgene_15069)

  • For your References section:

    Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo. Graff JM, Thies RS, Song JJ, Celeste AJ, Melton DA. Cell. 1994 Oct 7. 79(1):169-79. 10.1016/0092-8674(94)90409-X PubMed 7522972
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