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Addgene

pEBG p130cas
(Plasmid #15001)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15001 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone manufacturer
    Bruce Mayer Lab
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p130cas
  • Alt name
    p130 cas
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Bcar1 (a.k.a. Cas, Crkas, P130CAS)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    p130cas was originally from Mayer, and we made a GST fusion.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG p130cas was a gift from Raymond Birge (Addgene plasmid # 15001 ; http://n2t.net/addgene:15001 ; RRID:Addgene_15001)

  • For your References section:

    Activation of the focal adhesion kinase signaling pathway by structural alterations in the carboxyl-terminal region of c-Crk II. Zvara A, Fajardo JE, Escalante M, Cotton G, Muir T, Kirsch KH, Birge RB. Oncogene. 2001 Feb 22. 20(8):951-61. 10.1038/sj.onc.1204173 PubMed 11314030
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