pIRES2-EGFP Mer
(Plasmid #14998)

• Depositing Lab: Raymond Birge
• Publication: Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mer
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• Entrez Gene: Mertk (a.k.a. Eyk, Me, Mer, Ny, Nyk, nmf1, nmf12)
• Tag / Fusion Protein:
  • IRES EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.