CyPet-A206K-PRL2
(Plasmid
#149685)
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PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 149685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-25b-HRV3C-Cter8his
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCyPet-A206K-PRL2
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SpeciesH. sapiens (human), Synthetic
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MutationCyPet A206K
- Promoter T7
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Tag
/ Fusion Protein
- 8 histidine tag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: NdeI, 3' cloning site: XhoI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CyPet-A206K-PRL2 was a gift from Motoyuki Hattori (Addgene plasmid # 149685 ; http://n2t.net/addgene:149685 ; RRID:Addgene_149685) -
For your References section:
A FRET-based screening method to detect potential inhibitors of the binding of CNNM3 to PRL2. Cai F, Huang Y, Wang M, Sun M, Zhao Y, Hattori M. Sci Rep. 2020 Jul 30;10(1):12879. doi: 10.1038/s41598-020-69818-x. 10.1038/s41598-020-69818-x PubMed 32733084
