pGL2 (basic) 3xARE Lux
(Plasmid #14934)

• Depositing Lab: Joan Massague
• Publication: Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Backbone

• Vector backbone: pGL2 Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xARE
• Alt name: 3ARE-Lux
• Alt name: activin responsive region
• Insert Size (bp): 400
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The A3-Luc reporter construct was generated by subcloning the region comprising the three AREs and the core promoter from the previously described A3-CAT construct (Huang et al. 1995, EMBO J 14(23):5965-5973.) into the pGL2-Basic luciferase vector (Promega).