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pcDNA3.4_CEACAM6-His
(Plasmid #149337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 149337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.4_mIgG2-mMxr8a
  • Backbone manufacturer
    Diamond lab
  • Backbone size w/o insert (bp) 7472
  • Total vector size (bp) 6320
  • Modifications to backbone
    original plasmid digested with EcoRI/AgeI to remove portion of prior insert leaving IL-2 signal peptide sequence and C-terminal polyhistidine tag.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CEACAM6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    858
  • Mutation
    point mutation (GGA to GCA) to replace 'G-glycine' with 'A-alanine' at the site of lipidation required for GPI anchoring at plasmid position 1772
  • Entrez Gene
    CEACAM6 (a.k.a. CD66c, CEAL, NCA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • polyhistidine (C terminal on insert)
    • IL-2 signal peptide (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAAGTCTTGCACTTGTCACGAATTCGATATCGAAGCTCACTATTGAATCCACG
  • 3′ sequencing primer CTCAATGGTGATGGTGATGATGACCGGTTGCAGAGACTGTGATCATCGTG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    original CEACAM6 clone was obtained from pRC202454 OriGene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.4_CEACAM6-His was a gift from James Fleckenstein (Addgene plasmid # 149337 ; http://n2t.net/addgene:149337 ; RRID:Addgene_149337)

  • For your References section:

    CEACAMs serve as toxin-stimulated receptors for enterotoxigenic Escherichia coli. Sheikh A, Tumala B, Vickers TJ, Alvarado D, Ciorba MA, Bhuiyan TR, Qadri F, Singer BB, Fleckenstein JM. Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29055-29062. doi: 10.1073/pnas.2012480117. Epub 2020 Nov 2. 10.1073/pnas.2012480117 PubMed 33139570
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