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pSG424 Smad2 (Gal4-Smad2)
(Plasmid #14932)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG424
  • Backbone manufacturer
    Mark Ptashne
  • Backbone size w/o insert (bp) 3400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Smad2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • GenBank ID
    AF027964
  • Entrez Gene
    SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
  • Tag / Fusion Protein
    • Gal4 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An EcoRI fragment of Human Smad2 encoding 21nts 5'UTR, the coding region of Smad2, and 50nts of 3'UTR was inserted into the EcoRI site of pSG424.

The author's map shows the empty pSG424 map, which was described in Sadowski and Ptashne (1989) Nucleic Acids Research Vol 17 (18): p7539.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG424 Smad2 (Gal4-Smad2) was a gift from Joan Massague (Addgene plasmid # 14932 ; http://n2t.net/addgene:14932 ; RRID:Addgene_14932)

  • For your References section:

    Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. Liu F, Pouponnot C, Massague J. Genes Dev. 1997 Dec 1. 11(23):3157-67. 10.1101/gad.11.23.3157 PubMed 9389648
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