pTRE-TIGHT miR-1 (aka:pTRE-m1d)
(Plasmid #14896)

• Depositing Lab: David Bartel
• Publication: Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Backbone

• Vector backbone: pTRE-TIGHT
• Backbone manufacturer: Bartel Lab
• Backbone size w/o insert (bp): 2600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: miR-1
• Alt name: miR-1d
• Alt name: miR1
• Species: H. sapiens (human)
• Insert Size (bp): 500

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: ClaI (unknown if destroyed)
• 5′ sequencing primer: pLNCX primer

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression of a transcript behind TRE-Tight tetracycline regulatable promoter. This vector is part of the BD-Tet-ON/Tet-Off system for inducible expression in mammalian cells.

miR-1 is predicted to target Glucose-6 phosphate 1-dehydrogenase (G6PD).

The sequence provided is approximated based on the information that miR-1 was inserted at the BamHI and ClaI sites of pTRE-TIGHT. There may be slight differences in the actual sequence near the cloning sites.