MSCV-beta-catenin-IRES-GFP
(Plasmid #14717)

• Depositing Lab: Tannishtha Reya
• Publication: Reya et al Nature. 2003 May 22. 423(6938):409-14.

Backbone

• Vector backbone: MSCV-IRES-GFP
• Backbone size w/o insert (bp): 6573
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin
• Alt name: beta-catenin deltaGSK-KT3
• Species: M. musculus (mouse)
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII blunt (unknown if destroyed)
• 3′ cloning site: SalI/XhoI (destroyed during cloning)
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: IRES-R

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry