pT7-EGFP-C1-HsPatL1_I
(Plasmid
#146547)
-
PurposeMammalian Expression of HsPatL1
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 146547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneUnknown
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHsPatL1
-
SpeciesH. sapiens (human)
-
Entrez GenePATL1 (a.k.a. Pat1b, hPat1b)
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF extracted from NCBI: NM_152716.2 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT7-EGFP-C1-HsPatL1_I was a gift from Elisa Izaurralde (Addgene plasmid # 146547 ; http://n2t.net/addgene:146547 ; RRID:Addgene_146547) -
For your References section:
The C-terminal alpha-alpha superhelix of Pat is required for mRNA decapping in metazoa. Braun JE, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder O, Izaurralde E. EMBO J. 2010 Jul 21. 29(14):2368-80. 10.1038/emboj.2010.124 PubMed 20543818