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Addgene

Mouse ZP3 promoter (JD#445)
(Plasmid #14634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZP3 promoter
  • Alt name
    MoZP3p-MCS-BGHpA
  • Alt name
    ZP3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6800
  • Entrez Gene
    Zp3 (a.k.a. Zp-, Zp-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site I-SceI (MnSce I) (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer pBR322ori-F (5'-GGGAAACGCCTGGTATCTTT-3')
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse ZP3 promoter (JD#445) was a gift from Jurrien Dean (Addgene plasmid # 14634 ; http://n2t.net/addgene:14634 ; RRID:Addgene_14634)

  • For your References section:

    Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Rankin TL, Tong ZB, Castle PE, Lee E, Gore-Langton R, Nelson LM, Dean J. Development. 1998 Jul . 125(13):2415-24. PubMed 9609824
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