pMX Vav1 Y3F/CSH3* (P833L) uGFP
(Plasmid
#14566)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMX-uGFP
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVav1
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SpeciesH. sapiens (human)
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MutationY3F/CSH3* (P833L)
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Entrez GeneVAV1 (a.k.a. VAV)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMX-uGFP was a gift of Eugene Koh
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX Vav1 Y3F/CSH3* (P833L) uGFP was a gift from Joan Brugge (Addgene plasmid # 14566 ; http://n2t.net/addgene:14566 ; RRID:Addgene_14566) -
For your References section:
An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand. Wilsbacher JL, Moores SL, Brugge JS. Cell Commun Signal. 2006 . 4():5. 10.1186/1478-811X-4-5 PubMed 16709244