pMSCV neo KD Akt1
(Plasmid #14550)

• Depositing Lab: Joan Brugge
• Publication: Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34.

Backbone

• Vector backbone: pMSCV neo
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt1 KD
• Alt name: Akt1
• Species: M. musculus (mouse)
• Mutation: Kinase Dead Mutant: K179M
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Tag / Fusion Protein:
  • HA tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MSCV
• 3′ sequencing primer: MSCV rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Philip Tsichlis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry