-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepMSCV neo
-
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6500
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAkt1
-
SpeciesM. musculus (mouse)
-
Entrez GeneAkt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
-
Tag
/ Fusion Protein
- HA tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV
- 3′ sequencing primer MSCV rev (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byPhilip Tsichlis
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
wild-type Akt1
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMSCV neo WT Akt1 was a gift from Joan Brugge (Addgene plasmid # 14549 ; http://n2t.net/addgene:14549 ; RRID:Addgene_14549) -
For your References section:
Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. Irie HY, Pearline RV, Grueneberg D, Hsia M, Ravichandran P, Kothari N, Natesan S, Brugge JS. J Cell Biol. 2005 Dec 19. 171(6):1023-34. 10.1083/jcb.200505087 PubMed 16365168