pRSET FLIPmal-225u
(Plasmid
#14481)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSET
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLIPmal
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Alt nameFLIPmalE W62A
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Alt nameMBP
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SpeciesE. coli
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Insert Size (bp)2700
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MutationW62A
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET FLIPmal-225u was a gift from Wolf Frommer (Addgene plasmid # 14481 ; http://n2t.net/addgene:14481 ; RRID:Addgene_14481) -
For your References section:
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Fehr M, Frommer WB, Lalonde S. Proc Natl Acad Sci U S A. 2002 Jul 23. 99(15):9846-51. 10.1073/pnas.142089199 PubMed 12097642