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Addgene

pTGPD/P82
(Plasmid #1219)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1219 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRS314
  • Backbone manufacturer
    atcc
  • Backbone size w/o insert (bp) 8600
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hsp82
  • Alt name
    hsp82
  • Alt name
    hsp90
  • Alt name
    heat shock
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3100
  • Mutation
    GPD promoter [pTGPD] marked plasmid, select for with TRP1
  • GenBank ID
    NC_001148
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CIN2, which is adjacent to hsp82 on the yeast chromosome, was also picked up in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTGPD/P82 was a gift from Susan Lindquist (Addgene plasmid # 1219 ; http://n2t.net/addgene:1219 ; RRID:Addgene_1219)
  • For your References section:

    Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797