mRFP-Rab7
(Plasmid #14436)

• Purpose: Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
• Depositing Lab: Ari Helenius
• Publication: Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Backbone

• Vector backbone: pmRFP-C3
• Backbone size w/o insert (bp): 4658
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab 7
• Alt name: Rab7
• Species: Canis familialis
• Insert Size (bp): 663
• Mutation: 3 silent mutations near the C-terminus
• GenBank ID: M35522
• Entrez Gene: RAB7A (a.k.a. RAB7)
• Tag / Fusion Protein:
  • mRFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: 5'-GACTACACCATCGTGGAA

Resource Information

• Articles Citing this Plasmid:
  • 33 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry