Cav1-mRFP
(Plasmid #14434)

• Depositing Lab: Ari Helenius
• Publication: Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Backbone

• Vector backbone: pmRFP-N1
• Backbone size w/o insert (bp): 4687
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caveolin-1
• Alt name: Cav1
• Species: Canis familalis
• Insert Size (bp): 534
• GenBank ID: NM_001003296
• Entrez Gene: CAV1
• Tag / Fusion Protein:
  • mRFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (destroyed during cloning)
• 5′ sequencing primer: 5'-CCAAAATGTCGTAACAACTCCG

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the depositing laboratory recommends using CAV1-mCherry (Addgene Plasmid #27705) instead of CAV1-mRFP due to improved properties of the mCherry fluorophore.