pAG303GPD-ccdB-HA
(Plasmid
#14230)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS303
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Vector typeYeast Expression ; Gateway Destination
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsDB3.1, ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 3HA (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GPDPro_F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that this plasmid is missing the Cyc1 terminator. It is unknown how this will affect gene expression.
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG303GPD-ccdB-HA was a gift from Susan Lindquist (Addgene plasmid # 14230 ; http://n2t.net/addgene:14230 ; RRID:Addgene_14230) -
For your References section:
A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Alberti S, Gitler AD, Lindquist S. Yeast. 2007 Oct . 24(10):913-9. 10.1002/yea.1502 PubMed 17583893