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E. coli ER1821ΔlacI
(Bacterial strain #141407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 141407 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    30°C
  • Growth Strain(s)
    E. coli ER1821ΔlacI
  • Growth instructions
    The strain ER1821ΔlacI can be grown at 37°C. For the I-Block assay growth at 30°C is recommended.
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

λ- F- glnX44 e14- (McrA-) rfbD1 endA1 thi-1 Δ(yjiT-opgB)114::IS10 (EcoKI R- M- McrBC- Mrr-) + rpoS393(am) creC510 lrhA::IS3 ydeN::IS10 ΔlacI

Verification of lacI deletion:
PCR reaction on genomic DNA using AK362 and AK365 primers produces a 1936 bp fragment
AK 362 5’-CAATACCAATCGCACGCGG
AK 365 5’-CGAGACGTCACGGAAAATGCC

Phenotype: Constitutive β-galactosidase synthesis

This strain was derived from the precursor strain, E. coli ER1821, which is described in Jobling et al. (2016) Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems. Genome Announc 4(4):e00763-16. https://www.ncbi.nlm.nih.gov/pubmed/27516504

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli ER1821ΔlacI was a gift from Antal Kiss (Addgene plasmid # 141407)

  • For your References section:

    I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins. Szentes S, Zsibrita N, Koncz M, Zsigmond E, Salamon P, Pletl Z, Kiss A. Nucleic Acids Res. 2020 Mar 18;48(5):e28. doi: 10.1093/nar/gkaa014. 10.1093/nar/gkaa014 PubMed 31980824
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