XClone-Cas9-Cronus-T2A-BFP
(Plasmid
#141209)
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PurposeTunable and temporal expression control of Cas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneXLone-GFP
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9
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Alt nameCas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
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SpeciesStreptococcus pyogenes
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byXiaojun Lian (Addgene plasmid # 96930)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
derivatives of Addgene plasmid # 96930
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XClone-Cas9-Cronus-T2A-BFP was a gift from Rory Johnson (Addgene plasmid # 141209 ; http://n2t.net/addgene:141209 ; RRID:Addgene_141209)
