B-MFF
(Plasmid #141158)

• Purpose: Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-C1
• Modifications to backbone: AcGFP was replaced with ddGFP B (addgene plasmid #61017) using NheI/BspEI
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitochondrial fission factor
• Alt name: MFF, isoform 1
• Species: H. sapiens (human)
• Insert Size (bp): 1029
• GenBank ID: NM_020194
• Entrez Gene: MFF (a.k.a. C2orf33, EMPF2, GL004)
• Promoter: CMV
• Tag / Fusion Protein:
  • GB (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• Supplemental Documents:
  • B-MFF-CMV-Forward.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry