mito-mScarlet
(Plasmid #141153)

• Purpose: Fluorescent labeling of the mitochondrial matrix in mammalian cells
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4664
• Total vector size (bp): 4730
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: COX4
• Alt name: pre-CoxIV
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 66
• GenBank ID: NM_001181052
• Entrez Gene: COX4 (a.k.a. YGL187C)
• Promoter: CMV
• Tag / Fusion Protein:
  • mScarlet (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• Supplemental Documents:
  • mito-mScarlet.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry