mMaple-OMP25
(Plasmid #141151)

• Purpose: Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4689
• Total vector size (bp): 4809
• Modifications to backbone: AcGFP was replaced with mMaple
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OMP25
• Species: R. norvegicus (rat)
• Mutation: OMP25 c terminus from addgene plasmid #69598
• GenBank ID: NM_022599.2
• Entrez Gene: Synj2bp (a.k.a. Omp25)
• Promoter: CMV
• Tag / Fusion Protein:
  • mMaple (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: addgene plasmid #69598

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry