pC1-CMV-mito-DAAO
(Plasmid #141132)

• Purpose: Expresses DAAO with mitochondrial localization signal in mammalian cells
• Depositing Lab: Vsevolod Belousov
• Publication: Mammalian expression plasmid for production of DAAO in the mitochondrial matrix (unpublished)

Backbone

• Vector backbone: pC1
• Backbone size w/o insert (bp): 3952
• Total vector size (bp): 5248
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mito-DAAO
• Species: Rhodotorula gracilis (yeast)
• Insert Size (bp): 1296
• Mutation: deleted DAAO amino acids 367-368
• Promoter: CMV
• Tags / Fusion Proteins:
  • 2 mitochondrial localization signals (N terminal on insert)
  • 7xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CCA TTG ACG TCA ATG GGA GTT
• 3′ sequencing primer: CAA GTT AAC AAC AAC AAT TGC AT

Resource Information

• Supplemental Documents:
  • pC1-CMV-mito-DAAO.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry