pEGFP NFAT5 DBD
(Plasmid #14112)

• Depositing Lab: Anjana Rao
• Publication: Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

Backbone

• Vector backbone: pEGFP
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFAT5
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: DNA binding domain aa 175 to 471
• Entrez Gene: NFAT5 (a.k.a. NF-AT5, NFATL1, NFATZ, OREBP, TONEBP)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

DNA binding domain mutant, dominant negative mutant. Also called DBD5.