pC1-CMV-DAAO-NES
(Plasmid #141107)

• Purpose: Expresses DAAO with nuclear export signal in mammalian cells
• Depositing Lab: Vsevolod Belousov
• Publication: Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):117-127. doi: 10.1002/cpch.20.

Backbone

• Vector backbone: pC1
• Backbone size w/o insert (bp): 3982
• Total vector size (bp): 5110
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DAAO-NES
• Species: Rhodotorula gracilis (yeast)
• Insert Size (bp): 1128
• Mutation: deleted DAAO amino acids 367-368
• Promoter: CMV
• Tag / Fusion Protein:
  • Nuclear export signal (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CCA TTG ACG TCA ATG GGA GTT
• 3′ sequencing primer: CAA GTT AAC AAC AAC AAT TGC AT

Resource Information

• Supplemental Documents:
  • pC1-CMV-DAAO-NES.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry