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Addgene

pC1 Grx1-roCherry-mito
(Plasmid #141069)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141069 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pC1
  • Backbone size w/o insert (bp) 3919
  • Total vector size (bp) 5194
  • Modifications to backbone
    Grx1-roCherry was inserted into pC1 backbone, which was previously equipped with MLS1 and MLS2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grx1-roCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1275
  • Mutation
    Changed Alanine 150 to Cysteine, inserted Threonine after Cysteine 150, changed Serine 151 to Glutamate, changed Lysine 203 to Cysteine
  • Promoter CMV
  • Tag / Fusion Protein
    • dMTS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CCA TTG ACG TCA ATG GGA GTT
  • 3′ sequencing primer CAA GTT AAC AAC AAC AAT TGC AT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1 Grx1-roCherry-mito was a gift from Dmitry Bilan (Addgene plasmid # 141069 ; http://n2t.net/addgene:141069 ; RRID:Addgene_141069)
  • For your References section:

    Red fluorescent redox-sensitive biosensor Grx1-roCherry. Shokhina AG, Kostyuk AI, Ermakova YG, Panova AS, Staroverov DB, Egorov ES, Baranov MS, van Belle GJ, Katschinski DM, Belousov VV, Bilan DS. Redox Biol. 2019 Feb;21:101071. doi: 10.1016/j.redox.2018.101071. Epub 2018 Dec 7. 10.1016/j.redox.2018.101071 PubMed 30576927