Skip to main content
Addgene

pCS2+ Grx1-roCherry
(Plasmid #141068)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4072
  • Total vector size (bp) 5149
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grx1-roCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1077
  • Mutation
    Changed Alanine 150 to Cysteine, inserted Threonine after Cysteine 150, changed Serine 151 to Glutamate, changed Lysine 203 to Cysteine
  • Promoter CMV, SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TCGGAGCAAGCTTGATTTAGGTGACACTATA
  • 3′ sequencing primer GCATTCTAGTTGTGGTTTGTCCAAACTCATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Grx1-roCherry was a gift from Dmitry Bilan (Addgene plasmid # 141068 ; http://n2t.net/addgene:141068 ; RRID:Addgene_141068)

  • For your References section:

    Red fluorescent redox-sensitive biosensor Grx1-roCherry. Shokhina AG, Kostyuk AI, Ermakova YG, Panova AS, Staroverov DB, Egorov ES, Baranov MS, van Belle GJ, Katschinski DM, Belousov VV, Bilan DS. Redox Biol. 2019 Feb;21:101071. doi: 10.1016/j.redox.2018.101071. Epub 2018 Dec 7. 10.1016/j.redox.2018.101071 PubMed 30576927
Related items:
Commonly requested with: