pAdEasy Flag GCN5
(Plasmid #14106)

• Depositing Lab: Pere Puigserver
• Publication: Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Backbone

• Vector backbone: pAdEasy
• Backbone size w/o insert (bp): 25000
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCN5
• Species: H. sapiens (human)
• Mutation: E189G and K195N
• Entrez Gene: KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).

AdEasy is a registered trademark of the Johns Hopkins University.