pPB-tetpA-hNEUROG2-iRPT
(Plasmid #140764)

• Purpose: Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2
• Depositing Lab: Hideyuki Okano
• Publication: Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 10.1016/j.neures.2019.09.005.

Backbone

• Vector backbone: pPB-MCSfw-pA
• Total vector size (bp): 9558
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iRFP713
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 948

Gene/Insert 2

• Gene/Insert name: PuroR
• Alt name: Puromycin N-acetyl transferase
• Species: Streptomyces alboniger
• Insert Size (bp): 597

Gene/Insert 3

• Gene/Insert name: rtTAM2
• Insert Size (bp): 747

Gene/Insert 4

• Gene/Insert name: NEUROG2
• Species: H. sapiens (human)
• Insert Size (bp): 819
• GenBank ID: NM_024019.4
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)

Resource Information

• Supplemental Documents:
  • pPB-tetpA-hNEUROG2-iRPT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry